Upregulated CD133 expression in tumorigenesis of colon cancer cells

Figure 2 Fluorescence-activated cell sorting showing CD133 expression in SW620, CD133^- and CD133^hi cells (A) and in their spheroids (B), CD133 staining in spheroids of SW620, CD133^- and CD133^hi cells (original magnification × 100, brown indicates positive staining) (C), and reverse transcription-polymerase chain reaction showing CD133 expression in SW620, CD133^- and CD133^hi cells and their spheroids. ^aP < 0.05 vs monolayer cells. SP: Spheroid.