Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells

Table 1 Representative subsets of differentially expressed mast-cell genes after bacterial stimulation classified into functional categories

Go class	Description	P value						Gene examples
		LGG	Lc705	PJS	Bb12	Combination	Cpn
Upregulation
GO:0002376	Immune system process	3.48E-16	2.95E-13	-	5.25E-7	5.66E-12	1.64E-4	TNF, IL1B, IL8, IL10, CCL2, TLR1, TLR6, NOD2
GO:0043067	Regulation of programmed cell death	2.77E-8	9.63E-8	-	7.18E-4	3.49E-6	-	CASP3, CASP8, CDKN1
GO:0045321	Leukocyte activation	8.97E-9	2.17E-4	-	-	2.23E-4	-	CD8A, LCP2
Downregulation
GO:0007049	Cell cycle	4.76E-5	9.61E-7	-	-	5.81E-4	-	CCNA2, MAPK12
GO:0033033	Regulation of mast cell activation	-	7.51E-4	-	-	-	-	FCER1A, FCER1G
GO:0008610	Lipid biosynthetic process	-	-	-	2.02E-5	-	-	LPGAT1

The three selected highly upregulated or downregulated functional categories from the enrichment analysis performed with GOrilla Gene Enrichment Analysis and Visualization Tool. P value is the enrichment P value reported by GOrilla. The resultant categories reflect the gene expression differences of the bacteria-stimulated sample compared to unstimulated sample in 24 h time point. LGG: Lactobacillus rhamnosus GG; Lc705: Lactobacillus rhamnosus Lc705; PJS: Propionibacterium freudenreichii ssp. shermanii JS; Bb12: Bifidobacterium animalis ssp. lactis Bb12; Cpn: Chlamydia pneumoniae isolate Kajaani 6; TNF: Tumor necrosis factor; IL-1B: Interleukin-1β; IL-8: Interleukin-8; IL-10: Interleukin-10; CCL2: Chemokine (C-C motif) 2; TLR1: Toll-Like receptor 1; TLR6: Toll-Like receptor 6; NOD2: Nucleotide-binding oligomerization domain-containing protein 2; CASP3: Caspase 3; CASP8: Caspase 8; CDKN1: Cyclin-dependent kinase inhibitor 1; CD8A: Cluster of differentiation 8 A (T cell surface glycoprotein); LCP2: Lymphocyte cytosolic protein 2; CCNA2: Cyclin A2; MAPK12: Mitogen-activated protein kinase 12; FCER1A: Fc fragment of IgE high affinity I receptor for α polypeptide; FCER1G: Fc fragment of IgE high affinity I receptor for γ polypeptide; LPGAT1: Lysophosphatidylglycerol acyltransferase 1.