Bacteriocinogeny in experimental pigs treated with indomethacin and Escherichia coli Nissle

Table 1 DNA primers used for polymerase chain reaction detection of colicin encoding genes

Bacteriocin type	Primer name	5'-sequence-3'	Length of PCR product (nt)
A	ColA-F	CGTGGGGAAAAGTCATCATC
	ColA-R	GCTTTGCTCTTTCCTGATGC	475
B	colicinB-F	AAGAAAATGACGAGAAGACG
	colicinB-R	GAAAGACCAAAGGCTATAAGG	493
D	ColD-F	CTGGACTGCTGCTGGTGATA
	ColD-R	GAAGGTGCGCCTACTACTGC	420
E1	colicinE1-F	TGTGGCATCGGGCGAGAATA
	colicinE1-R	CTGCTTCCTGAAAAGCCTTTT	650
E2	ColE2-F	TGATGCTGCTGCAAAAGAG
	ColE2-R	TTCAAAGCGTTCCCTACCAC	409
E3	ColE3-F	TAAGCAGGCTGCATTTGATG
	ColE3-R	TCGGATCTGGACCTTTCAAC	413
E4	ColE4-F	GAAGGCTGCATTTGATGCT
	ColE4-R	CGGATCCGGACCTTTAATTT	409
E5	ColE3-F	TAAGCAGGCTGCATTTGATG
	ColE5-R	TTGAATTCTCGAATCGTCCA	430
E6	ColE6-F	ACCGAACGTCCAGGTGTT
	ColE6-R	TTTAGCCTGTCGCTCCTGAT	399
E7	ColE7-F	GCATTCTGCCATCTGAAAT
	ColE7-R	CTTCTGCCCACTTTCTTTCG	431
E8	ColE3-F	TAAGCAGGCTGCATTTGATG
	ColE8-R	GACTGATTGGCTTGTCGTGA	449
E9	ColE3-F	TAAGCAGGCTGCATTTGATG
	ColE9-R	GACTTTTCTCCCTCCGACCT	418
Ia	ColIa-F	GCATGCAAATGACGCTCTTA
	ColIa-R	GAGGACGCCAGTTCTCTGTC	473
Ib	ColIb-F	AACGAGTGGGTCGATGATTC
	ColIb-R	CCTTTTCTGCGCTCGTATTC	464
Js	ColJs-F	TCAAAATGTTTGGGCTCCTC
	ColJs-R	TAATCTGCCCTGTCCCACTG	254
K	ColK-F	CAGAGGTCGCTGAACATGAA
	ColK-R	TCCGCTAAATCCTGAGCAAT	469
M	ColM-F	GCTTACCACTTCGCAAAACC
	ColM-R	GAGCGACTCTCCGATAATGC	429
N	ColN-F	AGCTTGGCGAGTATCTTGGA
	ColN-R	CAACACAGCCCCGAATAAAC	401
S4	ColS4-F	TATATGGCCCAACTGCTGGT
	ColS4-R	CGTAAGGACGGACACCTGTT	456
U	ColU-F	TGATTGCTGCGAGAAAAATG
	ColU-R	TCTGACAGCCTCTCCCTGTT	485
Y	ColY-F	GCAGGCAGAAAAGAACAAGG
	ColY-R	CGGACGTTATTTGCCTTCAT	477
5	Col5-F	CATTGGCAAAAGCGAAATCT
	Col5-R	TGCAACTCTGGAAACAATCG	443
10	Col10-F	GGTTACCGGATTTCCTGGAT
	Col10-R	TTCTAGATGCTTGGCCCACT	448

PCR: Polymerase chain reaction.