Osteopontin expression is associated with hepatopathologic changes in Schistosoma japonicum infected mice

Figure 4 Profiles of osteopontin mRNA (RT-PCR) and protein (Western blotting) expression. No. 1-5 represent the model group at week 6, 8, 10, 14, and 18, respectively; No. 6-10 represent the control group at week 6, 8, 10, 14, and 18, respectively. A: Expression of osteopontin and GAPDH mRNA over time, the 100-bp GAPDH mRNA fragment was used as an internal control; B: Expression of osteopontin and GAPDH protein over time, the 37-kDa GAPDH band was used as an internal control; C: The IOD of osteopontin/GAPDH was expressed as the mean ± SD. M: Marker; n = 10 at each time. OPN: Osteopontin; RT-PCR: Reverse transcription polymerase chain reaction; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; IOD: Integral optical density. ^aP < 0.05 vs control; ^bP < 0.05 vs previous.