Human intestinal acyl-CoA synthetase 5 is sensitive to the inhibitor triacsin C

Figure 2 Human acyl-CoA synthetase 5 is triacsin C sensitive in CaCo2 cells. A: CaCo2 cells were stably transfected with the full-length ACSL5 expressing pcDNA_DEST40 plasmid or empty control vector. Expression of N-terminal 6xHis-tagged hu-ACSL5 protein in CaCo2 was analyzed by Western blot using anti-ACSL5 and anti-His antibodies; B: Indirect immunofluorescence of N-terminal 6xHis-tagged hu-ACSL5 proteins, mitochondria, and nuclei in CaCo2 cells shows the mitochondrial localization of recombinant ACSL5 proteins. Original magnification × 400; C: Analysis of ACSL activity (pH 5-10) in hu-ACSL5 CaCo2 transfectants and controls (pcDNA_DEST40 CaCo2 transfectants and CaCo2 wild type) in the presence (white symbols) or absence (black symbols) of 25 μmol/L triacsin C. ACSL5: Acyl-CoA synthetase 5.