Inhibition of tumor angiogenesis by TTF1 from extract of herbal medicine

Figure 8 TTF1 decreases the gene and protein level of angiogenesis regulation factors. Western blotting analysis was used to determine the protein levels of VEGF, KDR, bFGF, COX-2 and HIF-1α. A: Tumor tissues were centrifuged at 12 000 g for 15 min and the supernatant (70 μg/lane) was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. Western blotting analysis was performed to detect the protein levels of VEGF, KDR, bFGF, COX-2 and HIF-1α. Different compounds and dosages were used as treatments in the HepG-2-transplanted nude mice as follows: sTTF1 (5 μmol/kg); mTTF1 (10 μmol/kg); lTTF1 (20 μmol/kg); apigenin (40 μmol/kg); and Con (control group treated with normal saline); B: The intensity of the VEGF, KDR, bFGF, COX-2 and HIF-1α protein bands were determined and normalized with β-actin’s intensity by using the ultraviolet crosslinkers imager and plotted (^aP < 0.05 vs control group); C-E: Quantitative real-time PCR was performed to determine the mRNA expression levels of VEGF, KDR, bFGF, COX-2 and HIF-1α (^aP < 0.05 vs control group).