Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma

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Nov 28, 2011 (publication date) through Nov 12, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Nov 28, 2011; 17(44): 4917-4921
Published online Nov 28, 2011. doi: 10.3748/wjg.v17.i44.4917

Table 1 Primers for methylation-specific polymerase chain reaction

Gene		Primer sequence (5'–3')
WIF-1
U	F	GGGTGTTTTATTGGGTGTATTGT
	R	AAAAAAACTAACACAAACAAAATACAAAC
M	F	CGTTTTATTGGGCGTATCGT
	R	ACTAACGCGAACGAAATACGA
RUNX-3
U	F	TTATGAGGGGTGGTTGTATGTGGG
	R	AAAACAACCAACACAAACACCTCC
M	F	TTACGAGGGGCGGTCGTACGCGGG
	R	AAAACGACCGACGCGAACGCCTCC
SFRP-1
U	F	GAGTTAGTGTTGTGTGTTTGTTGTTTTGT
	R	CCCAACATTACCCAACTCCACAACCA
M	F	GTGTCGCGCGTTCGTCGTTTCGC
	R	AACGTTACCCGACTCCGCGACCG
DKK-3
U	F	TTAGGGGTGGGTGGTGGGGT
	R	CTACATCTCCACTCTACACCCA
M	F	GGGGCGGGCGGCGGGGC
	R	ACATCTCCGCTCTACGCCCG

M: Methylated sequence; U: Unmethylated sequence; F: Forward primer; R: Reverse primer.

Citation: Liu JB, Qiang FL, Dong J, Cai J, Zhou SH, Shi MX, Chen KP, Hu ZB. Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma. World J Gastroenterol 2011; 17(44): 4917-4921
URL: https://www.wjgnet.com/1007-9327/full/v17/i44/4917.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i44.4917