Copyright
©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Nov 14, 2011; 17(42): 4718-4724
Published online Nov 14, 2011. doi: 10.3748/wjg.v17.i42.4718
Published online Nov 14, 2011. doi: 10.3748/wjg.v17.i42.4718
Table 1 Primer sets for nested methylation-specific polymerase chain reaction
| Genes | Primer sequence (5'-3') | ||
| APC | U | F | GTGTTTTATTGTGGAGTGTGGGTT |
| R | CCAATCAACAAACTCCCAACAA | ||
| M | F | TATTGCGGAGTGCGGGTC | |
| R | TCGACGAACTCCCGACGA | ||
| WIF-1 | U | F | GGGTGTTTTATTGGGTGTATTGT |
| R | AAAAAAACTAACACAAACAAAATACAAAC | ||
| M | F | CGTTTTATTGGGCGTATCGT | |
| R | ACTAACGCGAACGAAATACGA | ||
| RUNX3 | U | F | TTATGAGGGGTGGTTGTATGTGGG |
| R | AAAACAACCAACACAAACACCTCC | ||
| M | F | TTACGAGGGGCGGTCGTACGCGGG | |
| R | AAAACGACCGACGCGAACGCCTCC | ||
| DLC-1 | U | F | AAACCCAACAAAAAAACCCAACTAACA |
| R | TTTTTTAAAGATTGAAATGAGGGAGTG | ||
| M | F | CCCAACGAAAAAACCCGACTAACG | |
| R | TTTAAAGATCGAAACGAGCGAGCG | ||
| SFRP-1 | U | F | GAGTTAGTGTTGTGTGTTTGTTGTTTTGT |
| R | CCCAACATTACCCAACTCCACAACCA | ||
| M | F | GTGTCGCGCGTTCGTCGTTTCGC | |
| R | AACGTTACCCGACTCCGCGACCG | ||
| DKK | U | F | TTAGGGGTGGGTGGTGGGGT |
| R | CTACATCTCCACTCTACACCCA | ||
| M | F | GGGGCGGGCGGCGGGGC | |
| R | ACATCTCCGCTCTACGCCCG | ||
| E-cad | U | F | TAATTTTAGGTTAGAGGGTTATTGT |
| R | CCACCCCAATACTAAATCACAACA | ||
| M | F | TTAGGTTAGAGGGTTATCGCGT | |
| R | TAACTAAAAATTCACCTACCGAC |
- Citation: Liu JB, Zhang YX, Zhou SH, Shi MX, Cai J, Liu Y, Chen KP, Qiang FL. CpG island methylator phenotype in plasma is associated with hepatocellular carcinoma prognosis. World J Gastroenterol 2011; 17(42): 4718-4724
- URL: https://www.wjgnet.com/1007-9327/full/v17/i42/4718.htm
- DOI: https://dx.doi.org/10.3748/wjg.v17.i42.4718