αV integrin: A new gastrin target in human pancreatic cancer cells

Figure 1 Increased expression of α_V integrin in response to gastrin in Panc-1 cells. A: Results of probing a 96 genes array with samples from unstimulated Panc-1 cells (control) or cells stimulated with 100 nmol/L of gastrin for 48 h; B: Real time polymerase chain reaction (PCR) analysis of α_V integrin mRNA expression in Panc-1 cells. Cells were treated or not with gastrin for the time indicated. Total RNA was isolated and α_V integrin mRNA expression was determined by real time PCR as described Materials and Methods; C, D: Expression of α_V integrin protein was examined by Western analysis following treatment of the cells with gastrin for 24 h. Blots were also probed with an antibody against GAPDH to ensure equal loading of proteins. Representative data from 3 experiments are shown. Quantifications of three experiments are presented as mean ± SE. Significance was accepted at P≤ 0.05, ^aP < 0.05, ^bP < 0.01.