Fascin promotes the motility and invasiveness of pancreatic cancer cells

Figure 2 Fascin overexpression induces alteration of cell morphology and cytoskeleton. A: Immunohistochemical analysis of actin distribution in fascin-overexpressing cells and vector control cells (× 400). (a) MIA PaCa-2 Fascin cells were more polarized with elongated membrane projections. Actin filaments were distributed as bundles in the cytoplasm which protruded into membrane projections in MIA PaCa-2 Fascin cells. (b) MIA PaCa-2 Vector cells showed a diffuse actin distribution; B: Immunofluorescence analysis of actin distribution in fascin-overexpressing cells and vector control cells (× 400). (a) Actin accumulated in a polarized manner in MIA PaCa-2 Fascin cells, whereas (b) MIA PaCa-2 Vector cells demonstrated a diffuse actin distribution.