Fascin promotes the motility and invasiveness of pancreatic cancer cells

Figure 1 The selection and generation of fascin-overexpressing pancreatic cancer cells. A: Western blotting analysis of fascin expression in pancreatic cancer cell lines. Fascin protein was present in all pancreatic cancer cell lines at different expression levels. BxPC-3, AsPC-1, PC-1, PC-4 and PC-7 express fascin at a high level, whereas MIA PaCa-2 expresses fascin at a very low level. Actin served as a loading control; B: Immunohistochemical analysis of fascin expression in MIA PaCa-2 cells transfected with either pcDNA3-Fascin or pcDNA3-Vector. (a) MIA PaCa-2 Fascin represents the stable transfected fascin-expressing cell line; (b) MIA PaCa-2 Vector is the control cell line. Magnification is × 200.