Multiphoton microscopy: An introduction to gastroenterologists

Figure 2 Images of normal tissue (above) and cancer tissue (below) treated with AG2. Normal tissues were incubated in artificial cerebrospinal fluid (ACSF) for 4 h, and cancer tissues were incubated in ACSF for 4 h, after which AG2 uptake was monitored. Right-side images are bright-field images, left-side images are pseudocolored two-photon microscopy (TPM) images obtained after incubation with AG2 for 4 h. The TPM images were obtained at a depth of 100 μm by collecting the two-photon excited fluorescence spectra in the range of 520-620 nm on excitation with fs pulses at 780 nm. IR: Infrared.