Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma

Figure 1 Promoter methylation contributes to pentraxin 3 downregulation in esophageal squamous cell carcinoma cell lines. A: Pentraxin 3 (PTX3) mRNA expression was detected by reverse transcription polymerase chain reaction; B: The methylation status of PTX3 promoter was determined by methylation-specific polymerase chain reaction. C: Representative high-resolution analyses of the methylation status of individual CpG sites in the PTX3 promoter by bisulphite genomic sequencing. Each row represents one bacterial clone with one circle symbolizing one CpG site. Methylated and unmethylated CpG sites were indicated by black and white circles, respectively. PTX3: Pentraxin 3; GAPDH: Glyceraldehyde-3-phosohate dehydrogenase; MSP: Methylation-specific polymerase chain reaction; USP:Unmethylation.