Hepatic steatosis prevents heme oxygenase-1 induction by isoflurane in the rat liver

Figure 1 Hepatic heme oxygenase-1 gene expression and heme oxygenase enzyme activity in lean and obese Zucker rats after treatment with pentobarbital or isoflurane. A: Reverse transcriptase-polymerase chain reaction was performed for determination of HO-1 mRNA expression. Data are presented as mean ± SE of the mean for n = 5 per group. ^bP < 0.01 vs all other groups; ^cP < 0.05 vs LEAN+PEN; B: Western blotting analysis for determination of HO-1 protein expression in two representative animals from each group; C: Measurement of HO enzyme activity. Data are presented as mean ± SE for n = 5 per group. ^eP < 0.001 vs all other groups. HO-1: Heme oxygenase-1; PEN: Pentobarbital; ISO: Isoflurane. LEAN: Lean Zucker rats; FAT: Obese Zucker rats; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.