Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells

Figure 5 High-mobility group box 1 siRNA inhibited α-smooth muscle actin, collagen typesIand III mRNA expression in hepatic stellate cell-T6 cells. A: Real-time polymerase chain reaction (RT-PCR) analysis for α-smooth muscle actin (α-SMA), collagen typesIand III mRNA expression in hepatic stellate cell-T6 cells after siRNA high-mobility group box 1 transfection. β-actin was used as the internal loading control; B-D: Semiquantitative analysis of the RT-PCR result. ^aP < 0.05 vs negative controls (NC) or mock.