Corticotropin-releasing factor secretion from dendritic cells stimulated by commensal bacteria

Figure 1 Detection of corticotropin-releasing factor by enzyme-linked immunosorbent assay. JAWSII cells were treated with indicated bacteria for 2 h and conditioned medium was examined by enzyme-linked immunosorbent assay. Data represent the mean ± SEM of 8 independent experiments. Statistical analysis was carried out using analysis of variance and Fisher's protected least significant difference. ^aP < 0.0001 vs control. CRF: Corticotropin-releasing factor; C. clostrodiiforme: Clostridium clostrodiiforme; B. vulgatus: Bacteroides vulgatus; E. coli: Escherichia coli; F. varium: Fusobacterium varium.