Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma

Figure 5 Effect of azathioprine plus buthionine sulfoximine treatment on cellular signaling. A: Stress activated kinases from HepG2 cells pretreated with buthionine sulfoximine (BSO) (24 h) and then cotreated with different combinations of azathioprine (AZA) with SP600125, SB203580 or N-acetylcysteine (NAC) for 1 h; B: Time course of mitochondrial regulatory proteins from HepG2 cells pretreated with BSO (500 μmol/L, 24 h) and then treated with AZA (300 μmol/L) at different times. This set of experiments is representative of three others with similar results.