Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma

Figure 4 Analysis by flow cytometry of HepG2 cells apoptosis (annexin V) and of mitochondrial functionality (JC-1). Cells treated with dimethyl sulfoxide (DMSO) (24 h), or pretreated with buthionine sulfoximine (BSO) (24 h) and then cotreated with azathioprine (AZA) (24 h) in absence or presence of N-acetylcysteine (NAC). This experiment is representative of three others with similar results.