Preclinical evaluation of azathioprine plus buthionine sulfoximine in the treatment of human hepatocarcinoma and colon carcinoma

Table 2 Summary of enzyme levels in mice

Enzyme	DMSO	BSO	AZA	BSO/AZA
AST (IU/L)	91.07 ± 8.36	62.79 ± 3.89	124.90 ± 10.56	154.30 ± 19.75a
ALT (IU/L)	31.50 ± 9.12	22.36 ± 2.85	36.17 ± 5.44	42.29 ± 9.04
CK (IU/L)	598.60 ± 112.10	416.00 ± 89.28	904.40 ± 129.40	918.50 ± 128.70

Enzyme activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatine kinase (CK) in plasma of nude mice transplanted with HepG2 cells at the end of the experiment (12 d of treatment in similar conditions as in Figure 7). Significant differences with respect to control animals [dimethyl sulfoxide (DMSO)] were statistically analyzed by analysis of variance.

^aP < 0.001. BSO: Buthionine sulfoximine; AZA: Azathioprine.