Dickkopf3 overexpression inhibits pancreatic cancer cell growth in vitro

Figure 2 Dickkopf3 promoter methylation analysis in human pancreatic cancer cell lines. A: Methylation-specific PCR (MSP) was performed with bisulfite-treated DNA from pancreatic cancer cells. The Dickkopf3 (Dkk3) promoter was significantly methylated in MIA PaCa-2 and AsPC-1 cells; B: MSP controls demonstrate the specificity of the Dkk3 primers used. Methylated bisulfite-converted DNA exclusively yields amplification products with primers specific to methylated Dkk3 promoter sequences; unmethylated bisulfite-converted DNA yields exclusively amplification products with primers recognizing unmethylated Dkk3 promoter sequences. MD: Methylated bisulfite-converted DNA; UD: Unmethylated bisulfite-converted DNA; U: PCR products amplified with primers recognizing unmethylated Dkk3 promoter sequences; M: Amplification generated with methylation-specific primers.