Rebamipide promotes healing of colonic ulceration through enhanced epithelial restitution

Figure 4 Involvement of extracellular signal-regulated kinase and Rho in rebamipide-treated rat intestinal epithelial cells. A: Rat intestinal epithelial (RIE) cells were treated with rebamipide (2 mmol/L) with or without an extracellular signal-regulated kinase inhibitor (U01263, 10 μmol/L) after wound induction. The wound healing area (12 h later) was then monitored. Data represent the mean ± SE of four experiments. ^aP < 0.05 vs controls. ^bP < 0.05 vs the 2 mmol/Lrebamipide-treated group. B: Expression of p44/42 and phospho-p44/42 in RIE cells incubated with rebamipide (2 mmol/L) was measured using western blot analysis. Actin antibody was used as an internal control. Representative data from three observations is shown. C: RIE cells were treated with rebamipide (2 mmol/L) with or without Rho kinase inhibitor (Y27632, 1 μmol/L) after wound induction. The wound healing area (6 h later) was then monitored. Data represent the mean ± SE of four experiments. ^aP < 0.05 vs controls. ^bP < 0.05 vs the 2 mmol/L rebamipide-treated group. DMSO: Dimethyl sulfoxide.