Function of nonstructural 5A protein of genotype 2a in replication and infection of HCV with gene substitution

Figure 2 Fluorescence quantitative reverse transcription polymerase chain reaction assay for hepatitis C virus RNAs in cell lysis of RNA transcripts transfected Huh-7. 5 cells. Five micrograms in vitro transcribed RNAs were electroporated into 3 × 10⁵ Huh-7.5 cells. Transfected cells were harvested at the indicated time points after transfection. Intracellular hepatitis C virus (HCV) RNA was analyzed by fluorescence quantitative reverse transcription polymerase chain reaction and displayed as genome equivalents per microgram total RNA (GE/μg RNA).