Late SV40 factor: A key mediator of Notch signaling in human hepatocarcinogenesis

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Aug 7, 2011 (publication date) through Nov 28, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 7, 2011; 17(29): 3420-3430
Published online Aug 7, 2011. doi: 10.3748/wjg.v17.i29.3420

Table 1 Reverse transcription-polymerase chain reaction primer sequences

Primer	Sequence (5’-3’)	Product size (bp)	Temperature (°C)
Notch1-ICD	Forward: CTAAGATCTCCTGAGGGCTTCAAAGTGTC	2375	63
	Reverse: GCGAATTCCTTGAAGGCCTCCGGAAT
H-Ras (ORF)	Forward: GAGGATCCATGACGGAATATAAGCTGG	668	61
	Reverse: GTGAATTCTCAGGAGAGCACACACTTG
Hes-1	Forward: ATGAACGAGGTGACCCGCTT	443	62
	Reverse: CTGGAAGGTGACACTGCGTT
LSF (ORF)	Forward: GGAAAGATCTAGGATGGCCTGGGCTCTGAAG	1547	59
	Reverse: CAAAGCTTGGGCACGAAACGCCGCACTCCT
LSF (promoter analysis)	Forward: CTAGGTACCCAACATGGTAAGATCCTGTCTCT	2020	56
	Reverse: GGAGATCTTCTCATCCCTGCTTTCTGTTTCCT
GAPDH	Forward: GGTGGAGGTCGGAGTCAACGGA	240	60
	Reverse: GAGGGATCTCGCTCCTGGAGGA

ORF: Open-reading frame; ICD: Intracellular domain; LSF: Late SV40 factor; LSF (promoter analysis): Upstream sequence before start code.

Citation: Fan RH, Li J, Wu N, Chen PS. Late SV40 factor: A key mediator of Notch signaling in human hepatocarcinogenesis. World J Gastroenterol 2011; 17(29): 3420-3430
URL: https://www.wjgnet.com/1007-9327/full/v17/i29/3420.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i29.3420