Identification of methylation profile of HOX genes in extrahepatic cholangiocarcinoma

Table 3 HOXA2, HOXA5, HOXA11, HOXB4, and HOXD13 methylation status of 30 cholangiocarcinoma patients and 9 normal controls n (%)

Genes	Age (n = 39)			Normal (n = 9)	Patients (n = 30)			P value1
	< 60 yr (n = 11)	> 60 yr (n = 28)	P value		Tumor stageI/II (n = 3)	Tumor stage III/IV (n = 27)	P value
HOXA2	4 (7.69)	6 (15.39)	0.424	7 (77.78)	1 (33.33)	2 (7.41)	0.511	< 0.001
HOXA5	3 (7.69)	7 (17.95)	1.0	9 (100)	1 (33.33)	0 (0)	0.206	< 0.001
HOXA11	2 (5.13)	7 (17.95)	1.0	9 (100)	1 (33.33)	0 (0)	0.206	< 0.001
HOXB 4	3 (7.69)	5 (12.82)	0.663	6 (66.67)	2 (66.67)	0 (0)	0.007	0.001
HOXD13	4 (7.69)	6 (15.39)	0.424	8 (88.89)	1 (33.33)	1 (3.70)	0.193	< 0.001

Fisher exact test was performed to identify differences in median expression values within the tissue samples.

¹Fisher test between normal controls and cholangiocarcinoma patients.