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©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 7, 2011; 17(29): 3407-3419
Published online Aug 7, 2011. doi: 10.3748/wjg.v17.i29.3407
Published online Aug 7, 2011. doi: 10.3748/wjg.v17.i29.3407
Table 1 Primer sequences, fragments and annealing temperature used for bisulfite-polymerase chain reaction and reverse transcription-polymerase chain reaction
Genes | Primer 5'→3' | Annealing temperature (°C) | Size (bp) |
BSP | |||
HOXA2 | L: TGTTTTAATAGAATTTATGTGGTTGG | 56 | 190 |
R: ATAACTACCCTCTACCTCCCCC | |||
HOXA5 | L: GTTTTGGAGAAATATTATATAAAAGTTATT | 50 | 145 |
R: CAATTAAAAAAATAAATCCTACCC | |||
HOXA11 | L: TGAGTATAAGTATGTTGTATGGGGG | 60 | 148 |
R: TTATAACCACCTCAAAAAAAACAAC | |||
HOXB4 | L: TAGAGGTGAGGTAGAATAGGAGGGT | 60 | 204 |
R: ACCCAACACCAAAATTTACATAAAA | |||
HOXD13 | L: GTGGGTTTAGTTAGGTTTGGGT | 60 | 239 |
R: TCTAACCCTCTCTCCCTCTATAAAC | |||
RT-PCR | |||
HOXA2 | F: GTCACTCTTTGAGCAAGCCC | 59 | 345 |
R: TAGGCCAGCTCCACAGTTCT | |||
HOXA5 | F: ACTCCGGCAGGTACGGCTACG | 62 | 259 |
R: -CCGCTGGAGTTGCTTAGGGAG | |||
HOXA11 | F: TGCCAAGTTGTACTTACTACGTC | 61 | 181 |
R: GTTGGAGGAGTAGGAGTATGTA | |||
HOXB4 | F: GTGCAAAGAGCCCGTCGTCTA CC | 65 | 161 |
R: CGTGTCAGGTAGCGGTTGTAGTG | |||
HOXD13 | F: TGCTCCTCTTCTGCCGTTGT | 55 | 467 |
R: CCTGTGGCTGGTCCTTGGT |
- Citation: Shu Y, Wang B, Wang J, Wang JM, Zou SQ. Identification of methylation profile of HOX genes in extrahepatic cholangiocarcinoma. World J Gastroenterol 2011; 17(29): 3407-3419
- URL: https://www.wjgnet.com/1007-9327/full/v17/i29/3407.htm
- DOI: https://dx.doi.org/10.3748/wjg.v17.i29.3407