Doxycycline blocks gastric ulcer by regulating matrix metalloproteinase-2 activity and oxidative stress

Figure 4 Effect of doxycycline on matrix metalloproteinase-3 activity during protection against indomethacin-induced gastric ulcer. Gastric ulcer was induced by intragastric administration of 48 mg/kg indomethacin in rats, and doxycycline was given 30 min prior to indomethacin for protection studies. Casein zymography was performed using phosphate buffered saline (PBS) (A) and Triton X-100 (TX) (C) extracts of gastric tissue from different groups of rats. Histographic representation of arbitrary activity of pro-matrix metalloproteinase (MMP)-3 in PBS (B) and TX (D) extracts of gastric tissues as measured by Lab Image software designed using the above zymogram and two other representative zymograms from independent experiments. Data expressed as mean ± SE. ^aP < 0.05, vs indomethacin; ^dP < 0.001 vs control.