Doxycycline blocks gastric ulcer by regulating matrix metalloproteinase-2 activity and oxidative stress

Figure 2 Effect of doxycycline on secreted and synthesized matrix metalloproteinase-2 and -9 activities during protection against indomethacin-induced ulcers. Gastric ulcer was induced by intragastric administration of 48 mg/kg indomethacin in rats, and doxycycline was given 30 min prior to indomethacin for protection studies. Gelatin zymography was performed using phosphate buffered saline (PBS) (A) and Triton X-100 (TX) (C) extracts of gastric tissue from different groups of rats. Histographic representation of arbitrary activity of pro-matrix metalloproteinase (MMP)-9 (B) and proMMP-2 (D) in PBS and TX extracts of gastric tissues, as measured by Lab Image software using the above zymogram and two other representative zymograms from independent experiments. Data expressed as mean ± SE. ^aP < 0.05, ^bP < 0.01, ^dP < 0.001 vs control; ^eP < 0.05, ^fP < 0.01 vs indomethacin.