Copyright
©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 28, 2011; 17(28): 3300-3309
Published online Jul 28, 2011. doi: 10.3748/wjg.v17.i28.3300
Published online Jul 28, 2011. doi: 10.3748/wjg.v17.i28.3300
Figure 5 Ex vivo arginase activity of organs from arginase II knockout mice fed water or the arginase inhibitor S-(2-boronoethyl)-L-cysteine.
Mice were administered 20 μL water or 50 mmol/L S-(2-boronoethyl)-L-cysteine (BEC) once or twice daily for 3 d by direct oral delivery via pipetting. Animals were euthanized and organs removed and homogenized. The data represent the average arginase activity of four to five mice ± SD, with each mouse organ measured in duplicate or triplicate using arginine buffer at pH 9.0 in the presence of manganese. A: Liver. Arginase activity was inhibited almost 50% in the liver by BEC. P = 0.0006 between the two groups; B: Kidney. Arginase activity was inhibited > 75% in the kidney by BEC. P = 0.0033 between the two groups; C: Antrum. Arginase activity was inhibited > 75% in the antrum by BEC. P = 0.0007 between the two groups; D: Body. No inhibition was observed in the body (P > 0.05).
-
Citation: Kim SH, Langford ML, Boucher JL, Testerman TL, McGee DJ.
Helicobacter pylori arginase mutant colonizes arginase II knockout mice. World J Gastroenterol 2011; 17(28): 3300-3309 - URL: https://www.wjgnet.com/1007-9327/full/v17/i28/3300.htm
- DOI: https://dx.doi.org/10.3748/wjg.v17.i28.3300