Interferon-γ inhibits ghrelin expression and secretion via a somatostatin-mediated mechanism

Figure 4 The somatostatin receptor agonist octreotide inhibits activation of the ghrelin promoter. Pulmonary carcinoid NCI-H727 cells were transfected with a 2 kb mouse ghrelin promoter construct and treated for 24 h with forskolin, IBMX, octreotide, and interferon γ (IFNγ) alone or in combination, after which the ghrelin promoter response was measured by the dual-luciferase reporter assay system. Octreotide inhibited both basal and IBMX and/or forskolin-induced ghrelin expression. Forskolin and IBMX were dissolved in dimethyl sulfoxide (DMSO). ^aP < 0.05.