Cisplatin pretreatment enhances anti-tumor activity of cytokine-induced killer cells

Figure 6 Tumor microvessel density after combination therapy. BALB/c WT mice were injected s.c. with 1 × 10⁶ CT-26 cells and the treatment protocols were initiated 7 d later. On day 19, tumor sections were prepared and analyzed by CD31 staining (A). Ten individual fields (0.16 mm²) at × 400 magnification were chosen to assess the tumor microvessel density (B). The experimental groups consisted of five mice per group. Representative sections from all groups are shown. Scale bars: 25 μm; Columns: mean microvessel number; Bars: SE.