Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth

Figure 6 Effects of silence of signal transduction and activation of transcription 3 gene on the expression of CyclinD1 and Bcl-xL. A: Reverse transcription polymerase chain reaction analysis. The RNA samples (2 μg in each) extracted from SW1990 cells, SW1990 cells transfected with a control vector (SW1990-Con), and SW1990 cells transfected with STAT3-RNAi (SW1990-RNAi) were subjected to RT-PCR for CyclinD1, Bcl-xL and β-actin mRNAs. RT-PCR for β-actin was performed in parallel to show an equal amount of total RNA in the sample; B: Western blotting analysis. Whole protein extracts (100 μg in each) were prepared from SW1990 cells, SW1990-Con, and SW1990-RNAi. The expression of CyclinD1 protein was determined by Western blotting with an anti-CyclinD1 antibody. The expression of Bcl-xL protein was determined by Western blotting analysis with an anti-Bcl-xL antibody. The β-actin expression levels were determined as a control for equivalent protein loading. Results shown represent one of the three experiments.