Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth

Figure 5 Effects of silence of signal transduction and activation of transcription 3 gene on tumor growth in vivo. A: Tumor growth curve after injection with mock, pRNAT-Con, and pRNAT-STAT3-siRNA-II cells. Intratumoral electroinjection of pRNAT-STAT3-siRNA-II resulted in significant inhibition of tumor growth; Points: mean (n = 6); bars: SD. ^aP < 0.05 vs control; B: STAT3 expression of the tumors after injection with mock, pRNAT-Con, and pRNAT-STAT3-siRNA-IIcells was analyzed by Western blotting. In pRNAT-STAT3-siRNA-II–treated tumors, STAT3 expression was significantly reduced. β-actin expression served as a control for equivalent protein loading; C: Tumor sections obtained from mock-, pRNAT-Con-, and pRNAT-STAT3-siRNA-II cells –injected tumors were immunostained using anti-STAT3 antibody. In pRNAT-STAT3-siRNA-II–treated tumors, STAT3 expression was significantly reduced.