Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth

Figure 2 Effects of silence of signal transduction and activation of transcription 3 gene on cell proliferation and anchorage-independent growth of pancreatic cancer cells. A: Cell growth curve of pancreatic cancer cells. SW1990 cells and stably-transfected cells (SW1990-Con and SW1990-RNAi) were subjected to MTT assay as described in materials and methods. Cell proliferation of SW1990-RNAi cells was significantly reduced compared with parental SW1990 and SW1990-Con cells; B: Cell colony formation in soft agar of pancreatic cancer cells. SW1990 cells and stably-transfected cells (SW1990-Con and SW1990-RNAi) were subjected to colony formation assay as described in materials and methods. The anchorage-independent growth ability of SW1990-RNAi cells was significantly reduced compared with parental SW1990 and SW1990-Con cells. Columns: mean (n = 3); Bars: SD. ^aP < 0.05 vs control. SW1990-Con: SW1990 pRNAT-Con transfectants; SW1990-RNAi: SW1990 STAT3-RNAi transfectants.