CD133+ gallbladder carcinoma cells exhibit self-renewal ability and tumorigenicity

Figure 3 Chemoresistance assays of spheroid cells. Sphere-forming cells and differentiated cells were seeded in 96-well plates at 3000 cells/well. Chemotherapeutic reagents gemcitabine (1 μg/mL) and 5-fluorouracil (0.1 μg/mL) were added and cell survival was estimated by 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide assay. ^aP < 0.05 vs differentiated cells.