CD133+ gallbladder carcinoma cells exhibit self-renewal ability and tumorigenicity

Figure 1 Culture of floating spheres. Single primary gallbladder carcinoma cells were cultured in serum-free medium containing human epidermal growth factor (20 ng/mL) and basic fibroblast growth factor (10 ng/mL). A: After culture for 1 wk, non-adherent spheres were observable (original magnification × 200); B: The spheres were dissociated and were plated into the same stem-cell-selective medium; similar progeny spheres emerged after 2 wk (original magnification × 200); C: The second progeny spheres were derived from the first progeny sphere-forming cells (original magnification × 200).