Antitumor activity of mutant bacterial cytosine deaminase gene for colon cancer

Figure 5 Efficacy of wild-type bCD gene or bCD mutant D314A gene suicide gene therapy in human colon carcinoma xenografts. LoVo human colon carcinoma cells with different gene transfection (5 × 10⁶ cells/mouse) were injected into the right flank of athymic nude mice (10 mice/group). Two weeks after tumor cell injection, PBS or 5-fluorocytosine (5-FC) (500 mg/kg) was injected intraperitoneal once a day for 14 d. On the day after LoVo cell injection, mice were monitored daily for survival. bCD: Bacterial cytosine deaminase; bCDwt: wild-type bCD; bCD-D314A: bCD mutant D314A; LoVo/null: LoVo cell transfected with pLJM1-GFP; LoVo/bCDwt: LoVo cell transfected with pLJM1-bCDwt-GFP; LoVo/bCD-D314A: LoVo cell transfected with pLJM1-bCD-D314A-GFP.