α-fetoprotein involvement during glucocorticoid-induced precocious maturation in rat colon

Figure 3 Immunofluorescence localization of α-fetoprotein and vimentin in the suckling rats treated with or without hydrocortisone. Labeling by the α-fetoprotein (AFP) antibody was detected with a fluorescein isothiocyanate (green) labeled secondary antibody. Labeling of vimentin was detected with a rhodamine-(red)-labeled secondary antibody on the same section. The overlap of AFP (green) and vimentin (red) labeling displayed orange color. Double-staining revealed complete colocalization of AFP and vimentin in the same cells of rat colons treated with or without hydrocortisone. All of the primary magnifications are × 200.