Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA

Figure 4 Immunohistochemical staining (× 400) analysis of Chang Gung CCA cells (upper two panels, A-H) and xenograft tissues of CCA mouse models (lower panel, I-L). A: Negative control; B: Negative expression of K-ras; C: EGFR weakly expressed in a cytoplasmic distribution; D-G: Her-II, Biliary cytokeratin (CK19), COX-II, and Met diffusely expressed in a cytoplasmic distribution; H: MUC-4 strongly and diffusely expressed in a cytoplasmic and membranous distribution; I-L: The results revealed that CK-19, COX-II, Met, and MUC4 are diffusely expressed in a cytoplasmic distribution in the rat CCA xenograft.