Genomic imbalances in esophageal carcinoma cell lines involve Wnt pathway genes

Table 4 Fluorescence in situ hybridization for detection of CCND1 and C-MYC amplification

Cell line	FISH signals	Amplitude1	Log2Ratio
CCND1
WHCO1	5-15	2	1.56
WHCO3	> 20	2	2.57
WHCO5	10-20	1	0.78
WHCO6	4-8	1	0.55
SNO	15-20	1	0.79
C-MYC
WHCO1	4-6	1	0.12
WHCO3	15-> 20	2	1.83
WHCO5	10-> 20	2	1.83
WHCO6	10-> 20	2	2.06
SNO	4-> 20	2	1.71

¹Amplitude threshold where log2ratio < 0.1 = 0, log2ratio > 0.1 < 0.9 = 1 and log2ratio > 0.9 = 2 as determined by SNP array copy number analysis. FISH: Fluorescence in situ hybridization.