Effects of sargentgloryvine stem extracts on HepG-2 cells in vitro and in vivo

Figure 2 Apoptosis of sargentgloryvine stem extract-treated HepG-2 cells (× 400). HepG-2 and ECV304 cells were treated with sargentgloryvine stem extract (SSE) at a concentration of 45 mg/mL for 24 h. Flow cytometry showed that apoptosis rate was increased obviously compared with the non-treated control cells, P < 0.05; while ECV304 cells did not show obvious diversity, P > 0.05. Positive signals in nucleus were observed obviously in SSE-treated HepG-2 cells by TdT-Mediated dUTP Nick End Labeling (TUNEL) and acridine orange/ethidium bromide (AO/EB) assays.