miRNA studies in in vitro and in vivo activated hepatic stellate cells

Figure 8 Relative expression of tissue inhibitor of metalloproteinase-3 mRNA in rno-miR-146a-overexpressing hepatic stellate cell-2 cells. A: The graph depicts the relative changes in tissue inhibitor of metalloproteinase (TIMP)-3 mRNA of three rno-miR-146a-overexpressing clones detected by real-time polymerase chain reaction (PCR). The data represent the mean ± SE of two different passages for each clone (^aP≤ 0.005); B: Primary hepatic stellate cells were treated with 50 nmol/L miR-146a mimic, and the expression of TIMP-3 mRNA was analyzed by real-time PCR and expressed as fold change relative to mock controls. The data represent the mean ± SE of three independent experiments (^bP≤ 0.01). HSC: Hepatic stellate cell.