miRNA studies in in vitro and in vivo activated hepatic stellate cells

Figure 6 Regulation of miR-146a by nuclear factor-κB. A: Knock-down experiments using nuclear factor (NF)-κB siRNAs showed a reduced level of cellular NF-κB (RelA) protein; B: The nuclear level of NF-κB (RelA) was decreased and showed a diminished DNA binding activity. Depicted is a representative Western blotting and electrophoretic mobility shift assay from three independent experiments; C: Downregulation of NF-κB (RelA) mRNA due to NF-κB siRNA transfection was accompanied by a decrease in miR-146a after 24 h. The data represent the mean ± SE of two independent experiments (^aP≤ 0.05, ^bP≤ 0.01, ^cP≤ 0.001); D: Cyclooxygenase-2 (Cox-2) protein was upregulated after NF-κB siRNA transfection. Shown are a representative Western blotting and the densitometric analysis of six independent experiments (^aP≤ 0.05).