miRNA studies in in vitro and in vivo activated hepatic stellate cells

Figure 1 Primary hepatic stellate cells and over-expression of miR-146a in hepatic stellate cell-2 cell line. A: Bright-field image of 1 d cultivated primary hepatic stellate cells and the corresponding vitamin A autofluorescence image are shown. Scale bar represents 100 μm. Real-time polymerase chain reaction (PCR) for in vivo activated hepatic stellate cells from rats on normal (n = 2) and choline-deficient ethionine supplemented (CDE) diet (n = 4). The mean ± SE for each diet model is shown; B: A representative image for the over-expression of miR-146a as visualized by the reporter GFP is shown. Real-time PCR for three independent clones confirmed the expression of miR-146a. The data represent the mean ± SE of triplicate reactions. SMAA: Smooth muscle α-actin.