Enhanced proliferation, invasion, and epithelial-mesenchymal transition of nicotine-promoted gastric cancer by periostin

Figure 2 Relative expression of periostin mRNA 48 h after transfection by siRNA plasmid. The silencing effect of the transfection of periostin siRNA plasmid in SGC-7901 cells was confirmed by quantitative reverse transcription-polymerase chain reaction 48 h after transfection. Four representative results are shown (S1-S4). SiRNA-C indicates gastric cancer cells with control plasmid. Control indicates gastric cancer cells without transfection. ^aP < 0.05 vs control. The Y ordinate indicates the ratio of periostin mRNA normalized to that of house-keeping genes. Notes: siRNA-S1, siRNA-S2, siRNA-S3, siRNA-S4: four representative results of periostin siRNA transfection; siRNA-C: vacant siRNA-control; control: SGC-7901 cells without transfection.