Enhanced proliferation, invasion, and epithelial-mesenchymal transition of nicotine-promoted gastric cancer by periostin

Figure 1 COX-2 inhibitor NS398 showed inhibitory effects on nicotine (200 ng/mL)-induced periostin expression after 24 h of treatment. COX-2-specific inhibitor NS398 (10 μmol/L) was applied to SGC-7901 cells 6 h before nicotine (200 μg/mL) treatment. A: Western blotting bands of periostin protein in four groups of gastric cancer cells; B: Quantification analysis demonstrated significantly higher relative expression of periostin in the nicotine group than in the other three groups (P < 0.05). Notes: CON: Control; Nicotine: Treated with nicotine; NS398: Treated with COX-2-specific inhibitor NS398; Ni + NS: Combined treatment with nicotine and NS398.