Keratinocyte growth factor gene therapy ameliorates ulcerative colitis in rats

Figure 5 Immunohistochemistry of keratinocyte growth factor receptor in colon tissues. The expression of the keratinocyte growth factor receptor (KGFR) protein was confirmed by immunohistochemistry with a rabbit anti-rat KGFR antibody as the primary antibody and a horse radish peroxidase-conjugated goat anti-rabbit antibody as the secondary antibody, and the brown color was considered to be positive staining. The expression of KGFR elevated significantly in attenuated Salmonella typhimurium Ty21a strain carrying human keratinocyte growth factor gene (SPK) group which was consistent with the results of Western blotting, and KGFR is located mainly in epithelial lamina. A: Representative wound tissue from the SPK group; B: Representative wound tissue from attenuated Salmonella typhimurium Ty21a strain group; C: Representative wound tissue from control group; D: Representative wound tissue from normal group (× 200).