Helicobacter pylori neutrophil activating protein as target for new drugs against H. pylori inflammation

Figure 4 Neutrophil adhesion to endothelial cells. A: Neutrophil adhesion to endothelial cells. Black bars indicate neutrophil adhesion to endothelial cells prior to lipopolysaccharides (LPS) removal while grey bars indicate the adhesion after LPS removal. The data represent triplicates from at least three independent experiments. Error bars indicate standard deviation (SD). Statistical evaluation was performed by Mann-Whitney test. Significant differences with the control values are marked by (^bP < 0.001) bar 1, control, bar 2, formyl-met-leu-pro peptide (fMLP), positive control of the procedure, bar 3, Helicobacter pylori neutrophil activating protein (HPNAP)wt-hexa-histidine peptide (6xHis) effect on neutrophil adhesion to endothelial cells before and after LPS removal, bar 4, HPNAP58-144-6xHis effect before and after LPS removal, bar 5, HPNAP1-57-6xHis effect before and after LPS removal, bar 6, 6xHis effect; B: Effect of arabino galactan proteins (AGPs) on neutrophil adhesion to endothelial cells, bar 1, control, bar 2, fMLP, positive control of the procedure, bar 3, effect of AGPs, bar 4, HPNAPwt-6xHis effect before and after LPS removal, bar 5, HPNAPwt-6xHis and AGPs co-effect, before and after LPS removal, bar 6, HPNAP58-144-6xHis effect before and after LPS removal, bar 7, HPNAP58-144-6xHis and mastic gum extract co-effect, before and after LPS removal, bar 8, 6xHis effect.