Switching-on of serotonergic calcium signaling in activated hepatic stellate cells

doi:10.3748/wjg.v17.i2.164

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Jan 14, 2011 (publication date) through Aug 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 14, 2011; 17(2): 164-173
Published online Jan 14, 2011. doi: 10.3748/wjg.v17.i2.164

Table 2 Primers for quantitative reverse transcription-polymerase chain reaction

Name	Sequence	Accession code	Position	Product (bp)
5-HT_2A
(+)	5'-GGGTACCTCCCACCGACAT-3'	NM_	234-252	101
(-)	5'-TTTCCAGCAATGGTGAGAATAATC-3'	17254	334-311
5-HT_2B
(+)	5'-CGCCATCCCAGTCCCTATTA-3'	NM_	781-800	101
(-)	5'-AGAGCATGAAACTGCCAAAGC-3'	17250	881-861
IP₃R 1
(+)	5'-GCAGAAGCAGATTGGCTATG-3'	NM_	2072-2091	261
(-)	5'-GTCTCAATCAGGATGTCAGC-3'	1007235	2332-2313
IP₃R 2
(+)	5'-CAAGAAGTTCAGAGACTGCC-3'	NM_	396-415	295
(-)	5'-ACGCATGGCATTCTTCTCCA-3'	31046.3	690-671
IP₃R 3
(+)	5'-GATGTGGTGTTGCTGCAGAA-3'	NM_	390-409	137
(-)	5'-TTGTTGCTCTTCATGTGCAG-3'	13138.1	526-507
RyR 1
(+)	5'-CTGAATGTCTGCTCTCCAAG-3'	AC	35577-35596	112
(-)	5'-GAAGGGCAGAGAGACAAGAT-3'	165142.3	35688-35669
RyR 2
(+)	5'-ATGTAGGCTTCTTCCAGAGC-3'	XR_	11405-11414	136
(-)	5'-TGCAGTACCTTCTCTCCTGA-3'	8338.1	11540-11521
RyR 3
(+)	5'-TACCTTGCCTGGTACACAAC-3'	XM_001080527.1	13957-13976	123
(-)	5'-AGTCACAGATGACAGGATCG-3'		14079-14060
SERCA 1
(+)	5'-CCAAGGAGCCTCTTATCAGT-3'	NM_017254	2516-2535	111
(-)	5'-CCTCTGCATACAAGAACCAC-3'		2626-2607
SERCA 2
(+)	5'-AGTTCATCCGCTACCTCATC-3'	M 23114	2297-2316	119
(-)	5'-CACCAGATTGACCCAGAGTA-3'		2415-2396
SERCA 3
(+)	5'-CTCATGCAGAAGGAGTTCAC-3'	NM_172812	1563-1582	140
(-)	5'-CGCTCAATTACACTCTCAGG-3'		1702-1683
Calreticulin
(+)	5'-AGAAGACTGGGATGAACGAG-3'	NM_	683-701	109
(-)	5'-GTCCTCAGGCTTCTTAGCAT-3'	22399.1	791-772
Calsequestrin-2
(+)	5'-CAGATGGCTATGAGTTCCTG-3'	NM_	988-1007	118
(-)	5'-CAGTAAGCAACAAGCAGAGG-3'	17131.2	1105-1086
Calnexin
(+)	5'-GTGTTTGCTACTGGTCCTTG-3'	NM_	21-40	146
(-)	5'-ATGGAGGAGTGCTGGTATCT-3'	172008.1	166-147
TGF-β type 1 R
(+)	5'-ACCAGCTATTGCCCATAGAG-3'	L 26110	1011-1030	106
(-)	5'-GGCAGAATCATGTCTCACAG-3'		1116-1097
α-SMA
(+)	5'-GCAGAGCAAGAGGGATCCT-3'	X 06801	222-242	73
(-)	5'-CATGTCGTCCCAGTTGGTGAT-3'		294-274
Cav1.2 (α1c)
(+)	5'-GACCCGTAGGAGCACGTTTG-3'	NM_012517	2327-2346	71
(-)	5'-CCTCCCCGGTCAGGATCT-3'		2397-2380

5-HT: 5-hydroxytryptamine; SERCA: Sarcoplasmic/endoplasmic reticulum Ca²⁺ ATPase; α-SMA: α-smooth muscle actin; RyR: Ryanodine receptor; TGF: Transforming growth factor.

Citation: Park KS, Sin PJ, Lee DH, Cha SK, Kim MJ, Kim NH, Baik SK, Jeong SW, Kong ID. Switching-on of serotonergic calcium signaling in activated hepatic stellate cells. World J Gastroenterol 2011; 17(2): 164-173
URL: https://www.wjgnet.com/1007-9327/full/v17/i2/164.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i2.164