Protective effects of ACLF sera on metabolic functions and proliferation of hepatocytes co-cultured with bone marrow MSCs in vitro

Figure 4 Cytotoxic effects of liver failure serum on hepatocytes cultured alone vs co-cultured hepatocytes. A: Transmission electron microscopy (TEM) analysis of the homo-cultured hepatocytes (left panel) and hepatocytes co-cultured with mesenchymal stem cells (MSCs) (righ panel) in the presence of 60% of liver failure serum; B: Lactate dehydrogenase (LDH) release assay of hepatocytes was indicated in each group; C: Live/dead assay of homo-cultured hepatocytes (upper panel) and hepatocytes co-cultured with MSCs (lower panel) in the presence of 60% of liver failure serum. ^aIndicates significant difference vs Hep-N group; ^bIndicates significant difference vs homo-hepatocytes cultured by various concentrations of liver failure serum (Hep-F) group. Hep-N: Hepatocytes homo-cultured with normal serum; Co-N:Hepatocytes co-cultured with normal serum; Co-F: Liver failure serum.