Hepatotropic growth factors protect hepatocytes during inflammation by upregulation of antioxidative systems

Figure 4 No additional nuclear factor-κB activation by hepatotropic growth factors. Electrophoretic mobility shift assay for nuclear factor (NF)-κB after hepatocyte pretreatment with hepatotropic growth factors and subsequent stimulation with lipopolysaccharide (LPS)-containing cytokine mixture (CM) demonstrated that stimulation with growth factors combined (A) or individually (B) did not further increase NF-κB expression when compared to stimulation with LPS-containing CM alone. Super shift and competition analysis (C) of NF-κB. HGF: Hepatocyte growth factor; EGF: Epidermal growth factor; TGF: Transforming growth factor.